Satellite imageries have been used to assess the impact of urbanization on the natural ecosystem in the last 30 years in Shillong. Compared to 1989, the built-up area in 1999 increased by a drastic 13%, followed by 1% for 2009, and 3% for 2019. The analysis shows that during the last forty years, the built-up area increased by a significant 17%, taking up the area under the vegetation cover which decreased by the same percentage. It was observed that the Southern and the Eastern part of the city had a much longer increase in the built-up areas. According to the analysis, Ward nos. 1,2,5,8,22,23,26 and 27 have shown more than 50% increase in the built-up area over the last 40 years.